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  • 技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 實驗室常見熒光蛋白的激發(fā)波長和發(fā)射波長

    實驗室常見熒光蛋白的激發(fā)波長和發(fā)射波長

    更新時間:2023-04-06   點擊次數(shù):4143次

    紫外熒光蛋白UV Proteins

    ProteinExcitation Wavelength  Emission Wavelength       
    Sirius355424
    Sandercyanin375630
    shBFP-N158S/L173I375458




    藍(lán)色熒光蛋白Blue Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Azurite383450
    EBFP2383448
    mKalama1385456
    mTagBFP2                       399454
    TagBFP402457
    shBFP401458

    青色熒光蛋白Cyan Proteins

    ProteinExcitation WavelengthEmission Wavelength
    ECFP433475
    Cerulean433475
    mCerulean3433475
    SCFP3A433474
    CyPet435477
    mTurquoise434474
    mTurquoise2434474
    TagCFP458480
    mTFP1462492
    monomeric Midoriishi-Cyan470496
    Aquamarine430474

    綠色熒光蛋白Green Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TurboGFP482502
    TagGFP2483506
    mUKG483499
    Superfolder GFP485510
    Emerald487509
    EGFP488507
    Monomeric Azami Green   492505
    mWasabi493509
    Clover505515
    mNeonGreen506517
    NowGFP494502
    mClover3506518

    黃色熒光蛋白Yellow Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TagYFP508524
    EYFP513527
    Topaz514527
    Venus515528
    SYFP2515527
    Citrine516529
    Ypet517530
    lanRFP-ΔS83                      521592
    mPapaya1530541
    mCyRFP1528594

    桔色熒光蛋白Orange Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Monomeric Kusabira-Orange548559
    mOrange548562
    mOrange2549565
    mKOκ551563
    mKO2551565

    紅色熒光蛋白Red Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TagRFP555584
    TagRFP-T555584
    RRvT556583
    mRuby558605
    mRuby2559600
    mTangerine                            568585
    mApple568592
    mStrawberry574596
    FusionRed580608
    mCherry587610
    mNectarine558578
    mRuby3558592
    mScarlet569594
    mScarlet-I569593

    遠(yuǎn)紅熒光蛋白Far Red Proteins

    ProteinExcitation WavelengthEmission Wavelength
    mKate2588633
    HcRed-Tandem590637
    mPlum590649
    mRaspberry598625
    mNeptune600650
    NirFP605670
    TagRFP657                            611657
    TagRFP675598675
    mCardinal604659
    mStable597633
    mMaroon1609657
    mGarnet2598671

    近紅熒光蛋白Near IR Proteins

    ProteinExcitation WavelengthEmission Wavelength
    iFP1.4684708
    iRFP713 (iRFP)                     690713
    iRFP670643670
    iRFP682663682
    iRFP702673702
    iRFP720702720
    iFP2.0690711
    mIFP683704
    TDsmURFP642670
    miRFP670642670

    Sapphire-type Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Sapphire399511
    T-Sapphire                             399511
    mAmetrine406

    526

    Red Fluorescent Proteins

    Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

    The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

    Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


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